Characterization of Rabbit Mesenchymal Cell Attachment on Calcium Phosphate Surface

Vita Zalite, Marina Sokolova, Dmitrijs Jakovlevs, Karlis Rozenbergs, Liga Berzina-Cimdina


In the current study, the effect of three different treated surfaces of hydroxyapatite and β-tricalcium phosphate on mesenchymal cell attachment has been investigated. Calcium phosphate powders have been synthesized, uniaxially pressed, polished and sintered. Mesenchymal cells have been seeded onto unpolished, polished and polished-thermally etched ceramic samples. The ceramic samples have been characterized by XRD, FTIR and SEM. Results have shown that the best cell attachment and morphology are on the unpolished surface indicating that relatively rough surface is better for cell application.


Hydroxyapatite, implant surface, mesenchymal cells, β-tricalcium phosphate

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Lopez-Estebana, S., Saiza, E., Fujinob, S., et al. Bioactive Glass Coatings for Orthopedic Metallic Implants. Journal of the European Ceramic Society, 2003, vol. 23, pp. 2921–2930.

Ungersbock, A., Rahn, B. Methods to Characterize the Surface Roughness of Metallic Implants. Journal of Materials. Materials in Medicine, 1994, vol. 5, pp. 434-440.

Liua, X, Chub, P.K., Ding, Ch. Surface Modification of Titanium, Titanium Alloys and Related Materials for Biomedical Applications. Materials Science and Engineering, 2004, R 47, pp. 49-121.

Liu, D., Savino, K., Yates, M.Z. Coating of Hydroxyapatite Films on Metal Substrates by Seeded Hydrothermal Deposition. Surface & Coatings Technology, 2011, vol. 205, pp. 3975–3986.

De Groot, K., Wolke, J.G., Jansen, J.A. Calcium Phosphate Coatings for Medical Implants. Proceedings of the Institution of Mechanical Engineers. Part H, 1998, vol. 212, N 2, pp. 137-147.

Xu, L., Pan, F., Yu, G., Yang, L., Zhang, E., Yang, K. In vitro and In vivo Evaluation of the Surface Bioactivity of a Calcium Phosphate Coated Magensium alloy. Biomaterials, 2009, vol. 30, N 8, pp. 1512-1523.

Thanigaiarul, K., Elayaraja, K., Magudapathy, P., et al., Surface Modification of Nanocrystalline Calcium Phosphate Bioceramic by Low Energy Nitrogen Ion Implantation. Ceramics International, 2012,

Tastepe, C.S., Liu, Y., Visscher, C.M., et al. Cleaning and Modification of Intraorally Contaminated Titanium Discs with Calcium Phosphate Powder Abrasive Treatment. Clinical Oral Implants Research, 2012,

Symietz, Ch., Lehmann, E., Gildenhaar, R., et al. Mechanical Stability of Ti6Al4V Implant Material after Femtosecond Laser Irradiation. Journal of Applied Physics, 2012, vol. 112.

Hung, K.-Y., Lo, S.-Ch., Shih, Ch.-Sh., et al. Titanium Surface Modified by Hydroxyapatite Coating for Dental Implants. Surface Coating Technology, 2012.

Roohani-Esfahani, S.I., Nouri-Khorasani, S., Lu, Z.F. Modification of Porous Calcium Phosphate Surfaces with Different Geometries of Bioactive Glass Nanoparticles. Materials Science and Engineering: C, 2012, vol. 32, pp. 830–839.

García-Gareta, E., Hua, J., Knowles, J.C., et al. Comparison of Mesenchymal Stem Cell Proliferation and Differentiation between Biomimetic and Electrochemical Coatings on Different Topographic Surfaces. Journal of Materials. Materials in Medicine, 2012,

Ohgushi, H., Dohi, Y., Tamai, S., Tabata, S. Osteogenic Differentiation of Marrow Stromal Stem Cells in Porous Hydroxyapatite Ceramics. Journal of Biomedical Materials Research, 1993, vol. 27, pp. 1401–1407.

Oreffo, R.O.C., Driessens, F.C.M., Planell, J.A., et al. Growth and Differentiation of Human Bone Marrow Osteoprogenitors on Novel Calcium Phosphate Cements. Biomaterials, 1998, vol. 19, pp. 1845–1854.

Nishio, K., Neo, M., Akiyama, H., Nishiguchi, S., et al. The Effect of Alkali and Heat treated Titanium and Apatite Formed Titanium on Osteoblastic Differentiation of Bone Marrow Cells. Journal of Biomedical Materials Research, 2000, vol. 52, N 4, pp. 652–61.<652::AID-JBM9>3.0.CO;2-W

Anselme, K., Bigerelle, M., Noel, B., et al. Qualitative and Quantitative study of Human Osteoblast adhesion on Materials with Various Surface Roughnesses. Journal of Biomedical Materials Research, 2000, vol. 49, N 2, p. 155–166.<155::aid-jbm2>;2-j

Weibenbock, M., Stein, E., Undt, G., et al. Particle Size of Hydroxyapatite Granules Calcified from Red Algae Affects the Osteogenic Potential of Human Mesenchymal Stem Cells in vitro. Cells Tissues Organs, 2006, vol. 182, pp. 79–88.

Chen, F., Lam, W.M., Lin, C.J., et al. Biocompatibility of Electrophoretical Deposition of Nanostructured Hydroxyapatite Coating on Roughen Titanium Surface: in vitro Evaluation Using Mesenchymal Stem Cells. Journal of Biomedical Materials Research, 2007, vol. 82B, pp. 183–191.

Kivrak, N. and Cuneyt Tas, A., Synthesis of Calcium Hydroxyapatie-Tricalcium Phosphate (HA-TCP) Composite Bioceramic Powders and Their Sintering Behavior. Journal of American Ceramic Society, 1998, vol. 81, N 9, pp. 2245–2252.

Descamps, M., Rguiti, E., Tricoteaux, A., et al. Processing and Properties of Transparent Hydroxyapatite and β-tricalcium Phosphate Obtained by HIP Pocess. Ceramics International, 2013, vol. 39, pp. 283–288.

Dālmane, A., Histoloģija. Rīga : LU Akadēmiskais apgāds, 2004. 319 lpp.

DOI: 10.7250/msac.2014.004

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